Article
Reversible suppression of an essential gene in adult mice using transgenic RNA interference.
Proceedings of the National Academy of Sciences of the United States of America - 26 Apr 2011
McJunkin Katherine, Mazurek Anthony, Premsrirut Prem K, Zuber Johannes, Dow Lukas E, Simon Janelle, Stillman Bruce, Lowe Scott W
Abstract excerpt
RNAi has revolutionized loss-of-function genetics by enabling sequence-specific suppression of virtually any gene. Furthermore, tetracycline response elements (TRE) can drive expression of short hairpin RNAs (shRNAs) for inducible and reversible target gene suppression. Here, we demonstrate the feasibility of transgenic inducible RNAi for suppression of essential genes. We set out to directly target cell...
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