Article
Probing tumor phenotypes using stable and regulated synthetic microRNA precursors.
Nature genetics - 1 Nov 2005
Dickins Ross A, Hemann Michael T, Zilfou Jack T, Simpson David R, Ibarra Ingrid, Hannon Gregory J, Lowe Scott W
Abstract excerpt
RNA interference is a powerful method for suppressing gene expression in mammalian cells. Stable knock-down can be achieved by continuous expression of synthetic short hairpin RNAs, typically from RNA polymerase III promoters. But primary microRNA transcripts, which are endogenous triggers of RNA interference, are normally synthesized by RNA polymerase II. Here we show that RNA polymerase II promoters expressing...
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