Article
Controlled insertional mutagenesis using a LINE-1 (ORFeus) gene-trap mouse model.
Proceedings of the National Academy of Sciences of the United States of America - 16 Jul 2013
O'Donnell Kathryn A, An Wenfeng, Schrum Christina T, Wheelan Sarah J, Boeke Jef D
Abstract excerpt
A codon-optimized mouse LINE-1 element, ORFeus, exhibits dramatically higher retrotransposition frequencies compared with its native long interspersed element 1 counterpart. To establish a retrotransposon-mediated mouse model with regulatable and potent mutagenic capabilities, we generated a tetracycline (tet)-regulated ORFeus element harboring a gene-trap cassette. Here, we show that mice expressing tet-ORFeus...
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