Article
Overexpression of the relA gene in Escherichia coli.
The Journal of biological chemistry - 25 Feb 1991
Schreiber G, Metzger S, Aizenman E, Roza S, Cashel M, Glaser G
Abstract excerpt
Intracellular levels of guanosine 3',5'-bispyrophosphate (ppGpp) governed by the relA gene are normally regulated by aminoacyl-tRNA availability for protein synthesis. An experimental system is described in which cellular levels of ppGpp are controlled instead by induction of plasmid pKK223-3 der...
Topics
- Base Sequence
- Blotting, Western
- Electrophoresis, Gel, Two-Dimensional
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Guanosine Tetraphosphate
- Molecular Sequence Data
- Mutation
- Operon
- Plasmids
- Promoter Regions, Genetic
- Serine
- Transcription, Genetic