Article
Targeted genome modifications in soybean with CRISPR/Cas9.
BMC biotechnology - 12 Mar 2015
Jacobs Thomas B, LaFayette Peter R, Schmitz Robert J, Parrott Wayne A
Abstract excerpt
BACKGROUND: The ability to selectively alter genomic DNA sequences in vivo is a powerful tool for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA sequences in a number of organisms. Here, the CRISPR/Cas9 system is shown to be effective in soybean by knocking-out a green fluorescent protein (GFP) transgene and modifying nine endogenous loci. RESULTS: Targeted DNA mutations were detected in...
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