Article
The endogenous transposable element Tgm9 is suitable for generating knockout mutants for functional analyses of soybean genes and genetic improvement in soybean.
PloS one - 1 Jan 2017
Sandhu Devinder, Ghosh Jayadri, Johnson Callie, Baumbach Jordan, Baumert Eric, Cina Tyler, Grant David, Palmer Reid G, Bhattacharyya Madan K
Abstract excerpt
In soybean, variegated flowers can be caused by somatic excision of the CACTA-type transposable element Tgm9 from Intron 2 of the DFR2 gene encoding dihydroflavonol-4-reductase of the anthocyanin pigment biosynthetic pathway. DFR2 was mapped to the W4 locus, where the allele containing Tgm9 was termed w4-m. In this study we have demonstrated that previously identified morphological mutants (three chlorophyll...
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