Article
CRISPR/Cas9-Mediated Genome Editing in Soybean Hairy Roots.
PloS one - 1 Jan 2015
Cai Yupeng, Chen Li, Liu Xiujie, Sun Shi, Wu Cunxiang, Jiang Bingjun, Han Tianfu, Hou Wensheng
Abstract excerpt
As a new technology for gene editing, the CRISPR (clustered regularly interspaced short palindromic repeat)/Cas (CRISPR-associated) system has been rapidly and widely used for genome engineering in various organisms. In the present study, we successfully applied type II CRISPR/Cas9 system to generate and estimate genome editing in the desired target genes in soybean (Glycine max (L.) Merrill.). The single-guide...
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