Article
Targeted mutagenesis in soybean using the CRISPR-Cas9 system.
Scientific reports - 29 May 2015
Sun Xianjun, Hu Zheng, Chen Rui, Jiang Qiyang, Song Guohua, Zhang Hui, Xi Yajun
Abstract excerpt
Genome editing is a valuable technique for gene function analysis and crop improvement. Over the past two years, the CRISPR-Cas9 system has emerged as a powerful tool for precisely targeted gene editing. In this study, we predicted 11 U6 genes in soybean (Glycine max L.). We then constructed two vectors (pCas9-GmU6-sgRNA and pCas9-AtU6-sgRNA) using the soybean U6-10 and Arabidopsis U6-26 promoters, respectively,...
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