Article
Different host cell proteases activate the SARS-coronavirus spike-protein for cell-cell and virus-cell fusion.
Virology - 10 May 2011
Simmons Graham, Bertram Stephanie, Glowacka Ilona, Steffen Imke, Chaipan Chawaree, Agudelo Juliet, Lu Kai, Rennekamp Andrew J, Hofmann Heike, Bates Paul, Pöhlmann Stefan
Abstract excerpt
Severe acute respiratory syndrome coronavirus (SARS-CoV) poses a considerable threat to human health. Activation of the viral spike (S)-protein by host cell proteases is essential for viral infectivity. However, the cleavage sites in SARS-S and the protease(s) activating SARS-S are incompletely defined. We found that R667 was dispensable for SARS-S-driven virus-cell fusion and for SARS-S-activation by trypsin and...
Topics
- Cell Fusion
- Cell Line
- Furin
- Gene Expression Regulation, Viral
- Humans
- Leupeptins
- Mutation
- Peptide Hydrolases
- Severe acute respiratory syndrome-related coronavirus
- Trypsin
- Virus Internalization