Article
E3 ligase Rad18 promotes monoubiquitination rather than ubiquitin chain formation by E2 enzyme Rad6.
Proceedings of the National Academy of Sciences of the United States of America - 5 Apr 2011
Hibbert Richard G, Huang Anding, Boelens Rolf, Sixma Titia K
Abstract excerpt
In ubiquitin conjugation, different combinations of E2 and E3 enzymes catalyse either monoubiquitination or ubiquitin chain formation. The E2/E3 complex Rad6/Rad18 exclusively monoubiquitinates the proliferating cell nuclear antigen (PCNA) to signal for "error prone" DNA damage tolerance, whereas a different set of conjugation enzymes is required for ubiquitin chain formation on PCNA. Here we show that human E2...
Topics
- Binding, Competitive
- Blotting, Western
- Crystallography, X-Ray
- DNA Damage
- DNA-Binding Proteins
- Humans
- Models, Molecular
- Mutation
- Nuclear Magnetic Resonance, Biomolecular
- Proliferating Cell Nuclear Antigen
- Ubiquitin
- Ubiquitin-Conjugating Enzymes
- Ubiquitin-Protein Ligases