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Article

Direct genotyping of Cytochrome P450 2A6 whole gene deletion from human blood samples by the SmartAmp method.

Clinica chimica acta; international journal of clinical chemistry - 11 Jun 2011

Azuma Ken-Ichi, Lezhava Alexander, Shimizu Makiko, Kimura Yasumasa, Ishizu Yuri, Ishikawa Toshihisa, Kamataki Tetsuya, Hayashizaki Yoshihide, Yamazaki Hiroshi

Abstract excerpt

BACKGROUND: Genetic polymorphisms of the human CYP2A6 gene are considered to be a determinant of smoking behavior and tobacco-related lung cancer risk in male Japanese smokers. We developed a SmartAmp-based genotyping method to detect whole deletion of the CYP2A6 gene directly from blood samples without DNA isolation. METHODS: We validated the new method using CYP2A plasmids, 48 genomic DNA samples and 25 blood...

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PMID: 21420395
DOI: 10.1016/j.cca.2011.03.017

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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