A rapid multiplex PCR assay that can reliably discriminate the cytochrome P450 2D6 whole-gene deletion allele from 2D6*10 alleles.

Abstract excerpt

BACKGROUND: Genetic polymorphisms of the human CYP2D6 gene can affect the metabolism of many drugs in clinical use. As a first step toward identifying poor drug metabolizers in the clinical setting, we developed a new multiplex PCR-based genotyping method to detect CYP2D6 whole-gene deletion. METHODS: We validated the new method by analyzing 500 genomic DNA samples from a Japanese population with the conventional...

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• Asian People
• Cytochrome P-450 CYP2D6
• Cytochrome P-450 Enzyme System
• DNA Primers
• Gene Deletion