Article
Caspase cleavage of the Golgi stacking factor GRASP65 is required for Fas/CD95-mediated apoptosis.
Cell death & disease - 7 Oct 2010
Cheng J P X, Betin V M S, Weir H, Shelmani G M A, Moss D K, Lane J D
Abstract excerpt
GRASP65 (Golgi reassembly and stacking protein of 65 KDa) is a cis-Golgi protein with roles in Golgi structure, membrane trafficking and cell signalling. It is cleaved by caspase-3 early in apoptosis, promoting Golgi fragmentation. We now show that cleavage is needed for Fas-mediated apoptosis: expression of caspase-resistant GRASP65 protects cells, whereas expression of membrane proximal caspase-cleaved GRASP65...
Topics
- Amino Acid Sequence
- Apoptosis
- Caspases
- Fas Ligand Protein
- Golgi Apparatus
- Golgi Matrix Proteins
- HeLa Cells
- Humans
- Membrane Proteins
- Molecular Sequence Data
- Mutation