Article
Caspase-resistant Golgin-160 disrupts apoptosis induced by secretory pathway stress and ligation of death receptors.
Molecular biology of the cell - 1 Jun 2005
Maag Rebecca S, Mancini Marie, Rosen Antony, Machamer Carolyn E
Abstract excerpt
Golgin-160 is a coiled-coil protein on the cytoplasmic face of the Golgi complex that is cleaved by caspases during apoptosis. We assessed the sensitivity of cell lines stably expressing wild-type or caspase-resistant golgin-160 to several proapoptotic stimuli. Cells expressing a caspase-resistant mutant of golgin-160 were strikingly resistant to apoptosis induced by ligation of death receptors and by drugs that...
Topics
- Anisomycin
- Apoptosis
- Apoptosis Regulatory Proteins
- Autoantigens
- Caspases
- Enzyme Inhibitors
- Etoposide
- Fluorescent Antibody Technique, Indirect
- Golgi Matrix Proteins
- Green Fluorescent Proteins
- HeLa Cells
- Humans
- Kinetics