Article
The role of GRASP65 in Golgi cisternal stacking and cell cycle progression.
Traffic (Copenhagen, Denmark) - 1 Jun 2010
Tang Danming, Yuan Hebao, Wang Yanzhuang
Abstract excerpt
In vitro assays identified the Golgi peripheral protein GRASP65 as a Golgi stacking factor that links adjacent Golgi cisternae by forming mitotically regulated trans-oligomers. These conclusions, however, require further confirmation in the cell. In this study, we showed that the first 112 amino acids at the N-terminus (including the first PDZ domain, PDZ1) of the protein are sufficient for oligomerization....
Topics
- Brefeldin A
- Cell Cycle
- Cell Cycle Proteins
- Dimerization
- Golgi Apparatus
- Golgi Matrix Proteins
- HeLa Cells
- Humans
- Membrane Proteins
- Microscopy, Fluorescence
- Mitosis
- Mutation