Article
Measuring cooperative Rev protein-protein interactions on Rev responsive RNA by fluorescence resonance energy transfer.
RNA biology - 1 Jan 2000
Vercruysse Thomas, Pawar Sonalika, De Borggraeve Wim, Pardon Els, Pavlakis George N, Pannecouque Christophe, Steyaert Jan, Balzarini Jan, Daelemans Dirk
Abstract excerpt
The export of viral RNA from the nucleus to the cytoplasm of the cellular host is a crucial step in the life cycle of HIV-1 that is mediated by the viral Rev protein. One aspect of the Rev function, its multimerization, is still unexplored as a target for antiviral therapy. This is partly due to the lack of a fast and solid system to measure Rev multimerization. We have developed a high throughput in vitro Rev...
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