Article
SNaPshot and StripAssay as valuable alternatives to direct sequencing for KRAS mutation detection in colon cancer routine diagnostics.
The Journal of molecular diagnostics : JMD - 1 Mar 2011
Fariña Sarasqueta Arantza, Moerland Elna, de Bruyne Hanneke, de Graaf Henk, Vrancken Tamara, van Lijnschoten Gesina, van den Brule Adriaan J C
Abstract excerpt
Although direct sequencing is the gold standard for KRAS mutation detection in routine diagnostics, it remains laborious, time consuming, and not very sensitive. Our objective was to evaluate SNaPshot and the KRAS StripAssay as alternatives to sequencing for KRAS mutation detection in daily practice. KRAS exon 2-specific PCR followed by sequencing or by a SNaPshot reaction was performed. For the StripAssay, a...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.