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Article

KRAS mutation analysis in genomic DNA isolated from formalin-fixed paraffin-embedded ovarian tissue: evaluation of a strip-based reverse-hybridisation assay.

Journal of clinical pathology - 1 Mar 2011

Kriegshäuser Gernot, Auner Veronika, Schuster Eva, Holzer Barbara, Oberkanins Christian, Horvat Reinhard, Speiser Paul, Zeillinger Robert

Abstract excerpt

AIMS: To evaluate a reverse-hybridisation assay (strip assay) designed for the sensitive detection of 10 mutations in codons 12 and 13 of the KRAS gene. The strip assay relies on mutant-enriched PCR followed by reverse-hybridisation of biotinylated amplification products to oligonucleotide probes immobilised as an array of parallel lines on nitrocellulose test strips. METHODS: The strip assay was used to analyse...

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Source

Identifiers

PMID: 21258089
DOI: 10.1136/jcp.2010.081414

Genes

KRAS

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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