Improving the Limit of Detection for Sanger Sequencing: A Comparison of Methodologies for <i>KRAS</i> Variant Detection

Abstract excerpt

Fluorescent dye terminator Sanger sequencing (FTSS), with detection by automated capillary electrophoresis (CE), has long been regarded as the gold standard for variant detection. However, software analysis and base-calling algorithms used to detect mutations were largely optimized for resequencing applications in which different alleles were expected as heterozygous mixtures of 50%. Increasingly, the...

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