Article
Characterization of the promoter of the human Gi2 alpha-subunit gene.
Molecular endocrinology (Baltimore, Md.) - 1 Jul 1990
Weinstein L S, Kats I, Spiegel A M, Carter A D
Abstract excerpt
The promoter of the human gene for the alpha-subunit of Gi2, a GTP-binding signal transduction protein, was analyzed by cloning the 5' flanking region of the gene upstream of the bacterial chloramphenicol acetyltransferase (CAT) gene and measuring the level of CAT expression after transfection in CV-1 green monkey kidney cells. Analysis of multiple 5' deletion mutants reveals that a minimum of 85 bases upstream...
Topics
- Animals
- Base Sequence
- Cell Line
- Chloramphenicol O-Acetyltransferase
- Chlorocebus aethiops
- Cloning, Molecular
- DNA
- GTP-Binding Proteins
- Humans
- Molecular Sequence Data
- Mutation