Article
Expression of luteinising hormone-beta subunit chloramphenicol acetyltransferase (LH-beta-CAT) fusion gene in rat pituitary cells: induction by cyclic 3'-adenosine monophosphate (cAMP).
Molecular and cellular endocrinology - 1 Sept 1991
Clayton R N, Lalloz M R, Salton S R, Roberts J L
Abstract excerpt
In this study we determined the activity of the rat luteinising hormone-beta gene promoter in a heterologous rat pituitary cell line (GH3 cells). 1.7 kb of LH-beta 5' flanking sequence and the first 5 bp of the 5' untranslated region were ligated to the chloramphenicol acetyltransferase (CAT) rec...
Topics
- Adenylyl Cyclases
- Animals
- Base Sequence
- Cells, Cultured
- Chloramphenicol O-Acetyltransferase
- Cloning, Molecular
- Cyclic AMP
- DNA
- Gene Expression Regulation
- HeLa Cells
- Humans
- Luteinizing Hormone
- Molecular Sequence Data
- Mutation
- Organ Specificity
- Pituitary Gland
- Rats
- Recombinant Fusion Proteins