Article
Three-dimensional structure determination of an anti-2-phenyloxazolone antibody: the role of somatic mutation and heavy/light chain pairing in the maturation of an immune response.
The EMBO journal - 1 Dec 1990
Alzari P M, Spinelli S, Mariuzza R A, Boulot G, Poljak R J, Jarvis J M, Milstein C
Abstract excerpt
The three-dimensional structure of the Fab fragment of an anti-2-phenyloxazolone monoclonal antibody (NQ10/12.5) in its native and complexed forms has been determined at 2.8 and 3.0 A resolution, respectively. Identification of hapten-contacting residues has allowed us to evaluate the contributio...
Topics
- Amino Acid Sequence
- Antibodies, Monoclonal
- Crystallization
- Haptens
- Immunoglobulin Fab Fragments
- Immunoglobulin Heavy Chains
- Immunoglobulin Light Chains
- Ligands
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Oxazolone
- Protein Conformation
- X-Ray Diffraction