Article
Recombineering, transfection, Western, IP and ChIP methods for protein tagging via gene targeting or BAC transgenesis.
Methods (San Diego, Calif.) - 1 Apr 2011
Hofemeister Helmut, Ciotta Giovanni, Fu Jun, Seibert Philipp Martin, Schulz Alexander, Maresca Marcello, Sarov Mihail, Anastassiadis Konstantinos, Stewart A Francis
Abstract excerpt
Protein tagging offers many advantages for proteomic and regulomic research. Ideally, protein tagging is equivalent to having a high affinity antibody for every chosen protein. However, these advantages are compromised if the tagged protein is overexpressed, which is usually the case from cDNA expression vectors. Physiological expression of tagged proteins can be achieved by gene targeting to knock-in the protein...
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