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The pCri System: A Vector Collection for Recombinant Protein Expression and Purification

11 Nov 2014

Abstract excerpt

A major bottleneck in structural, biochemical and biophysical studies of proteins is the need for large amounts of pure homogenous material, which is generally obtained by recombinant overexpression. Here we introduce a vector collection, the pCri System, for cytoplasmic and periplasmic/extracellular expression of heterologous proteins that allows the simultaneous assessment of prokaryotic and eukaryotic host...

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PMID: 25386923
DOI: 10.1371/journal.pone.0112643

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