Article
Deglycosylation increases the fibrinolytic activity of a deletion mutant of tissue-type plasminogen activator.
Thrombosis and haemostasis - 28 Jun 1990
Wilhelm J, Kalyan N K, Lee S G, Hum W T, Rappaport R, Hung P P
Abstract excerpt
delta 2-89 t-PA is a deletion mutant lacking the finger (F) and epidermal growth factor (EGF) domains; thus, the fibrin interaction of this molecule must be mediated solely by the kringle region. In the present study, the influence of the oligosaccharide side-chains on the activity of delta 2-89...
Topics
- Amides
- Blood Coagulation Tests
- Cells, Cultured
- Enzyme-Linked Immunosorbent Assay
- Fibrinolysis
- Glycoside Hydrolases
- Glycosylation
- Mutation
- Neuraminidase
- Recombinant Proteins
- Tissue Plasminogen Activator