Article
Biochemical and functional characterization of human tissue-type plasminogen activator variants obtained by deletion and/or duplication of structural/functional domains.
The Journal of biological chemistry - 5 Apr 1990
Lijnen H R, Nelles L, Van Hoef B, De Cock F, Collen D
Abstract excerpt
Five cDNA encoding human tissue-type plasminogen activator (t-PA) variants with deletion and/or duplication of structural/functional domains were cloned and expressed in Chinese hamster ovary cells. The mutants included: rt-PA-delta FE (where r represents recombinant), with deletion of the finger...
Topics
- Affinity Labels
- Animals
- Cell Line
- Cloning, Molecular
- Cricetinae
- Cyanogen Bromide
- DNA
- DNA Restriction Enzymes
- Escherichia coli
- Fibrin
- Fibrinogen
- Fibrinolysis
- Gene Expression
- Humans
- Mutation
- Plasmids
- Sepharose
- Structure-Activity Relationship