Article
Kinetic characterization of tissue-type plasminogen activator (t-PA) and t-PA deletion mutants.
Thrombosis and haemostasis - 4 Mar 1991
de Vries C, Veerman H, Nesheim M E, Pannekoek H
Abstract excerpt
The binding of t-PA to fibrin is mediated both by its "finger" (F) and its "kringle 2" (K2) domain. In addition, these domains are involved in the stimulation of t-PA activity by fibrin. We analyzed the kinetic characteristics of Glu-plasminogen activation by t-PA and a set of t-PA deletion mutants in the absence and the presence of desA-fibrin. In the absence of desA-fibrin, the activity of t-PA (variants) is...
Topics
- Amino Acid Sequence
- Fibrin
- Kinetics
- Molecular Sequence Data
- Mutation
- Plasminogen
- Protein Binding
- Tissue Plasminogen Activator