Article
Disruption of interkringle disulfide bond of plasminogen kringle 1-3 changes the lysine binding capability of kringle 2, but not its antiangiogenic activity.
Archives of biochemistry and biophysics - 15 Mar 2000
Lee H, Kim H K, Lee J H, You W K, Chung S I, Chang S I, Park M H, Hong Y K, Joe Y A
Abstract excerpt
Kringle 1-3 of human plasminogen is a potent inhibitor of endothelial cell proliferation. To understand a possible role for the unique cystine bridge between kringle 2 and kringle 3, we disrupted the interkringle disulfide bond by mutating Cys(169) and Cys(297) to serine residues. The yield of the mutant during the refolding process was decreased significantly. Anti-endothelial cell proliferative activity of the...
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