Article
[A simple and convenient method for construction of gene site-directed mutagenesis].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics - 1 Apr 2002
Zhang Mingxiang, Wen Jian, Xia Kun, Zheng Duo, Zhang Zhuohua, Xia Jiahui
Abstract excerpt
OBJECTIVE: To introduce a new technique for rapid construction of gene site-directed mutagenesis. METHODS: Three primers are synthesized. One is a primer with the needed mutation; the other two containing appropriate enzyme sites for construction of the PCR fragment into a suitable plasmid are located at the flanks of the mutation primer. After the amplification of the PCR fragment using the mutation primer and...
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