Article
Transgenic mice with overexpression of mutated human optineurin(E50K) in the retina.
Molecular biology reports - 1 Feb 2012
Meng Qingfeng, Xiao Zheng, Yuan Huiping, Xue Fei, Zhu Yuanmao, Zhou Xinrong, Yang Binbin, Sun Jingbo, Meng Bo, Sun Xian, Cheng Fang
Abstract excerpt
In the present work, Site-directed mutagenesis to insert the Glu50Lys amino acid substitution was achieved by PCR using plasmid pBluescript-OPTN. Mutated human OPTN(E50K) gene-driven mouse c-kit promoter was constructed and confirmed by endonuclease digestion and sequence analysis. Transgenic mice were generated via the microinjection method. PCR and DNA dot blot were used to screen the positive transgenic mice....
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