Article
Expression of NANOG and NANOGP8 in a variety of undifferentiated and differentiated human cells.
The International journal of developmental biology - 1 Jan 2010
Ambady Sakthikumar, Malcuit Christopher, Kashpur Olga, Kole Denis, Holmes William F, Hedblom Emmett, Page Raymond L, Dominko Tanja
Abstract excerpt
The transcription factor NANOG is essential for maintaining pluripotency in embryonic stem cells. We have previously reported the expression of NANOG in adult human fibroblasts; here we present a more thorough investigation into the expression of NANOG in a panel of both differentiated and undifferentiated human cells. We utilize RT-PCR, qRT-PCR, cloning and sequencing, sequence alignment, restriction digestion,...
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