Site-directed mutagenesis of glycine-14 and two "critical" cysteinyl residues in Drosophila alcohol dehydrogenase.

doi:10.1021/bi00457a003

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Article

Site-directed mutagenesis of glycine-14 and two "critical" cysteinyl residues in Drosophila alcohol dehydrogenase.

Biochemistry - 6 Feb 1990

Chen Z, Lu L, Shirley M, Lee W R, Chang S H

Abstract excerpt

Three amino acid residues (glycine-14, cysteine-135, and cysteine-218) previously speculated to be important for the structure and function of Drosophila melanogaster alcohol dehydrogenase have been investigated by using site-directed mutagenesis followed by kinetic analysis and chemical modifica...

Topics

Alcohol Dehydrogenase
Animals
Cysteine
DNA
Dithionitrobenzoic Acid
Drosophila melanogaster
Enzyme Activation
Gene Expression
Glycine
Kinetics
Mutation
Polymerase Chain Reaction
Protein Denaturation

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