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Article

Effect of a conservative mutation of an active site residue involved in substrate binding on the hydride tunneling reaction catalyzed by choline oxidase.

Archives of biochemistry and biophysics - 1 Sept 2009

Quaye Osbourne, Gadda Giovanni

Abstract excerpt

The reaction of alcohol oxidation catalyzed by choline oxidase was previously shown to occur through the tunneling of a hydride ion from the alpha-carbon of an activated alkoxide species to the N(5) atom of FAD within a highly preorganized enzyme-substrate complex [G. Gadda, Biochemistry 47 (2008) 13745-13753]. In the present study, a glutamate residue (312) that participates in substrate binding has been...

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Identifiers

PMID: 19653994
DOI: 10.1016/j.abb.2009.07.021

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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