Article
Roles of Cys148 and Asp179 in catalysis by deoxycytidylate hydroxymethylase from bacteriophage T4 examined by site-directed mutagenesis.
Biochemistry - 27 Oct 1992
Graves K L, Butler M M, Hardy L W
Abstract excerpt
The proposed roles of Cys148 and Asp179 in deoxycytidylate (dCMP) hydroxymethylase (CH) have been tested using site-directed mutagenesis. CH catalyzes the formation of 5-(hydroxymethyl)-dCMP, essential for DNA synthesis in phage T4, from dCMP and methylenetetrahydrofolate. CH resembles thymidylat...
Topics
- Alleles
- Amino Acid Sequence
- Aspartic Acid
- Bacteriophage T4
- Cloning, Molecular
- Cysteine
- Deoxyuracil Nucleotides
- Escherichia coli
- Hydrogen Bonding
- Hydroxymethyl and Formyl Transferases
- Kinetics
- Mutagenesis, Site-Directed
- Recombinant Proteins
- Transferases