Article
Selective modification by transglutaminase of a glutamine side chain in the hinge region of the histidine-388----glutamine mutant of yeast phosphoglycerate kinase.
The Biochemical journal - 1 Jan 1991
Coussons P J, Kelly S M, Price N C, Johnson C M, Smith B, Sawyer L
Abstract excerpt
The transglutaminase-catalysed incorporation of putrescine and monodansylcadaverine into yeast phosphoglycerate kinase has been studied. There is little incorporation of the amines into wild-type enzyme, but nearly stoichiometric incorporation into the histidine-388----glutamine mutant enzyme. C....
Topics
- Amino Acid Sequence
- Catalysis
- Chromatography, High Pressure Liquid
- Circular Dichroism
- Electrophoresis, Polyacrylamide Gel
- Glutamine
- Histidine
- Molecular Sequence Data
- Mutation
- Peptide Mapping
- Phosphoglycerate Kinase
- Putrescine
- Saccharomyces cerevisiae
- Spectrophotometry, Ultraviolet