Article
Modifications of the active center of T4 thioredoxin by site-directed mutagenesis.
The Journal of biological chemistry - 25 Feb 1990
Joelson T, Sjöberg B M, Eklund H
Abstract excerpt
The active site sequence of T4 thioredoxin, Cys-Val-Tyr-Cys, has been modified in two positions to Cys-Gly-Pro-Cys to mimic that of Escherichia coli thioredoxin. The two point mutants Cys-Gly-Tyr-Cys and Cys-Val-Pro-Cys have also been constructed. The mutant proteins have similar reaction rates w...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Binding Sites
- Escherichia coli
- Genotype
- Kinetics
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Plasmids
- Protein Conformation
- Restriction Mapping
- T-Phages
- Thioredoxin-Disulfide Reductase
- Thioredoxins