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Article

Modifications of the active center of T4 thioredoxin by site-directed mutagenesis.

The Journal of biological chemistry - 25 Feb 1990

Joelson T, Sjöberg B M, Eklund H

Abstract excerpt

The active site sequence of T4 thioredoxin, Cys-Val-Tyr-Cys, has been modified in two positions to Cys-Gly-Pro-Cys to mimic that of Escherichia coli thioredoxin. The two point mutants Cys-Gly-Tyr-Cys and Cys-Val-Pro-Cys have also been constructed. The mutant proteins have similar reaction rates with T4 ribonucleotide reductase as has the wild-type T4 thioredoxin. Mutant T4 thioredoxins with Pro instead of Tyr at...

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PMID: 2406248

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