Article
A block at Man5GlcNAc2-pyrophosphoryldolichol in intact but not disrupted castanospermine and swainsonine-resistant Chinese hamster ovary cells.
The Journal of biological chemistry - 5 Feb 1990
Zeng Y C, Lehrman M A
Abstract excerpt
A mutation in glycoprotein processing inhibitor-resistant (PIR) Chinese hamster ovary (CHO) cells was previously shown to result in a block at the Man5GlcNAc2 stage of the dolichol-oligosaccharide biosynthetic pathway (Lehrman, M.A., and Zeng, Y. (1989) J. Biol. Chem. 264, 1584-1593). These cells had normal mannose-P-dolichol synthase activity and were able to transfer the Man5GlcNAc2 oligosaccharides to protein....
Topics
- Alkaloids
- Animals
- Cell Fusion
- Cell Line
- Cell Membrane
- Cricetinae
- Cricetulus
- Drug Resistance
- Female
- Genotype
- Glycoside Hydrolases
- Indolizines