Article
Binding of trans-dominant mutant Rev protein of human immunodeficiency virus type 1 to the cis-acting Rev-responsive element does not affect the fate of viral mRNA.
The New biologist - 1 Dec 1990
Benko D M, Robinson R, Solomin L, Mellini M, Felber B K, Pavlakis G N
Abstract excerpt
The binding of Rev protein of human immunodeficiency virus type 1 (HIV-1) to the cis-acting Rev-responsive element (RRE) was compared to the binding of a trans-dominant Rev mutant. RevBL, which inhibits Rev function. Rev and RevBL expressed in bacteria were purified and shown to bind in vitro to the RRE with similar affinities. The study of the RRE mutants indicated that Rev and RevBL bind to the same target...
Topics
- Gene Expression Regulation, Viral
- Gene Products, gag
- Gene Products, rev
- Genes, rev
- HIV-1
- Humans
- In Vitro Techniques
- Mutation
- Nucleic Acid Conformation
- RNA, Messenger
- RNA, Viral