Article
An efficient protocol for rare mutation genotyping in a large population.
Genetic testing - 1 Jan 1998
Zarbl H, Aragaki C, Zhao L P
Abstract excerpt
We introduce a method to efficiently detect rare mutations for individual subjects in a large population by pooling samples and retesting subgroups of positive pooled samples. We conducted computer simulations of this method and discovered that it seems efficient for mutation prevalences less than 0.1, regardless of the number of samples. The simulations also indicate that splitting the pooled samples into three...
Topics
- Computer Simulation
- DNA Mutational Analysis
- Gene Frequency
- Genetic Testing
- Genotype
- Humans
- Mutation
- Prevalence