Article
Resolving lineage assignation on Mycobacterium tuberculosis clinical isolates classified by spoligotyping with a new high-throughput 3R SNPs based method.
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases - 1 Oct 2010
Abadia Edgar, Zhang Jian, dos Vultos Tiago, Ritacco Viviana, Kremer Kristin, Aktas Elif, Matsumoto Tomoshige, Refregier Guislaine, van Soolingen Dick, Gicquel Brigitte, Sola Christophe
Abstract excerpt
We developed a new multiplexed-PCR assay to accurately classify Mycobacterium tuberculosis complex (MTC) isolates at the sublineage level by single nucleotide polymorphisms (SNPs). This method relies on 7 SNPs located in different genes of the MTC strains (recC, rec0, recR, ligB, ligC, alkA, and mgtC). Most of these genes are involved in replication, repair and recombination (3R) functions of M. tuberculosis...
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