Article
Intragenic complementation between Escherichia coli trp repressors with different defects in the tryptophan-binding pocket.
Gene - 1 Aug 1992
Storbakk N, Oxender D L, el-Gewely M R
Abstract excerpt
Site-directed mutagenesis of the trpR gene (encoding the trp repressor, TrpR) was used to replace Gly85 with tryptophan (Trp or W), in order to place Trp near its normal location in the L-tryptophan(L-W)-binding pocket. The resulting mutant protein (G85W) exhibits weak, but significant repressor...
Topics
- Alleles
- Bacterial Proteins
- Base Sequence
- Binding Sites
- DNA, Bacterial
- Escherichia coli
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Repressor Proteins
- Tryptophan