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Recombinant protein production driven by the tryptophan promoter is tightly controlled in ICONE 200, a new genetically engineered E. coli mutant.

Biotechnology and bioengineering - 20 Aug 2000

Chevalet L, Robert A, Gueneau F, Bonnefoy J Y, Nguyen T

Abstract excerpt

Batch processes for recombinant gene expression in prokaryotic systems should optimally comprise a growth phase with minimal promoter activity followed by a short phase favoring expression. The strong promoter of the tryptophan operon (Ptrp) gives high-level expression of recombinant proteins in E. coli. The inefficiency to control basal expression before induction is however a major obstacle towards the use of...

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PMID: 10862673
DOI: 10.1002/1097-0290(20000820)69:4<351::aid-bit1>3.0.co;2-g

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