Article
Selection and analysis of non-interactive mutants in the Escherichia coli tryptophan synthase alpha subunit.
Molecular & general genetics : MGG - 1 May 1992
Swift S, Kuhn J, Stewart G S
Abstract excerpt
The inherent infidelity of Taq DNA polymerase in the polymerase chain reaction was exploited to produce random mutations in the trp A gene. Screening of the resulting clones allowed selection of non-interactive mutant alpha subunits retaining their intrinsic catalytic activity. Two single changes...
Topics
- Amino Acids
- Base Sequence
- DNA Mutational Analysis
- Escherichia coli
- Molecular Sequence Data
- Mutation
- Plasmids
- Polymerase Chain Reaction
- Protein Conformation
- Recombination, Genetic
- Selection, Genetic
- Tryptophan Synthase