Article
Functional characterization of a cancer causing mutation in human replication protein A.
Molecular cancer research : MCR - 1 Jul 2010
Hass Cathy S, Gakhar Lokesh, Wold Marc S
Abstract excerpt
Replication protein A (RPA) is the primary ssDNA-binding protein in eukaryotes. RPA is essential for DNA replication, repair, and recombination. Mutation of a conserved leucine residue to proline in the high-affinity DNA binding site of RPA (residue L221 in human RPA) has been shown to have defects in DNA repair and a high rate of chromosomal rearrangements in yeast. The homologous mutation in mice was found to...
Topics
- Animals
- Cell Cycle
- DNA Damage
- DNA Repair
- DNA Replication
- Gene Knockdown Techniques
- HeLa Cells
- Humans
- Mice
- Mutation
- Phosphorylation
- Replication Protein A