Article
Green fluorescent protein-based halide indicators with improved chloride and iodide affinities.
FEBS letters - 22 Jun 2001
Galietta L J, Haggie P M, Verkman A S
Abstract excerpt
The green fluorescent protein YFP-H148Q is sensitive to halides by a mechanism involving halide binding and a shift in pK(a). However, a limitation of YFP-H148Q is its low halide sensitivity, with K(d)>100 mM for Cl(-). Indicators with improved sensitivities are needed for cell transport studies, particularly in drug discovery by high-throughput screening, and for measurement of Cl(-) concentration in subcellular...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.