Mechanism and cellular applications of a green fluorescent protein-based halide sensor.

doi:10.1074/jbc.275.9.6047

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Article

Mechanism and cellular applications of a green fluorescent protein-based halide sensor.

The Journal of biological chemistry - 3 Mar 2000

Jayaraman S, Haggie P, Wachter R M, Remington S J, Verkman A S

Abstract excerpt

We report the application of a targetable green fluorescent protein-based cellular halide indicator. Fluorescence titrations of the purified recombinant yellow fluorescent protein YFP-H148Q indicated a pK(a) of 7.14 in the absence of Cl(-), which increased to 7.86 at 150 mM Cl(-). At pH 7.5, YFP-...

Topics

3T3 Cells
Animals
Bacterial Proteins
Chlorides
Circular Dichroism
Colforsin
Cystic Fibrosis Transmembrane Conductance Regulator
Green Fluorescent Proteins
Hydrogen-Ion Concentration
Kinetics
Luminescent Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mice

Microscopy, Fluorescence

Mutation

Spectrometry, Fluorescence

Spectrophotometry

Transfection

Source

Open in PubMed

Identifiers

PMID: 10692389
DOI: 10.1074/jbc.275.9.6047

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026. Prepared record publication-10692389-2026-08-31.1. Manifest publication-graph-cohort-2026-08-31.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 31 Aug 2026.

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