Article
All-codon scanning identifies p53 cancer rescue mutations.
Nucleic acids research - 1 Nov 2010
Baronio Roberta, Danziger Samuel A, Hall Linda V, Salmon Kirsty, Hatfield G Wesley, Lathrop Richard H, Kaiser Peter
Abstract excerpt
In vitro scanning mutagenesis strategies are valuable tools to identify critical residues in proteins and to generate proteins with modified properties. We describe the fast and simple All-Codon Scanning (ACS) strategy that creates a defined gene library wherein each individual codon within a specific target region is changed into all possible codons with only a single codon change per mutagenesis product. ACS is...
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