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Article

One-Tube-Only Standardized Site-Directed Mutagenesis: An Alternative Approach to Generate Amino Acid Substitution Collections.

PloS one - 1 Jan 2016

Mingo Janire, Erramuzpe Asier, Luna Sandra, Aurtenetxe Olaia, Amo Laura, Diez Ibai, Schepens Jan T G, Hendriks Wiljan J A J, Cortés Jesús M, Pulido Rafael

Abstract excerpt

Site-directed mutagenesis (SDM) is a powerful tool to create defined collections of protein variants for experimental and clinical purposes, but effectiveness is compromised when a large number of mutations is required. We present here a one-tube-only standardized SDM approach that generates comprehensive collections of amino acid substitution variants, including scanning- and single site-multiple mutations. The...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 27548698
DOI: 10.1371/journal.pone.0160972
PMCID: PMC4993582

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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