Article
T-cell receptor microclusters critical for T-cell activation are formed independently of lipid raft clustering.
Molecular and cellular biology - 1 Jul 2010
Hashimoto-Tane Akiko, Yokosuka Tadashi, Ishihara Chitose, Sakuma Machie, Kobayashi Wakana, Saito Takashi
Abstract excerpt
We studied the function of lipid rafts in generation and signaling of T-cell receptor microclusters (TCR-MCs) and central supramolecular activation clusters (cSMACs) at immunological synapse (IS). It has been suggested that lipid raft accumulation creates a platform for recruitment of signaling molecules upon T-cell activation. However, several lipid raft probes did not accumulate at TCR-MCs or cSMACs even with...
Topics
- Adaptor Proteins, Signal Transducing
- Animals
- CD3 Complex
- Endocytosis
- Fluorescence Resonance Energy Transfer
- Green Fluorescent Proteins
- In Vitro Techniques
- Lymphocyte Activation
- Macromolecular Substances
- Membrane Microdomains
- Membrane Proteins
- Mice