Article
De novo phosphorylation and conformational opening of the tyrosine kinase Lck act in concert to initiate T cell receptor signaling.
Science signaling - 17 Jan 2017
Philipsen Lars, Reddycherla Amarendra V, Hartig Roland, Gumz Janine, Kästle Matthias, Kritikos Andreas, Poltorak Mateusz P, Prokazov Yury, Turbin Evgeny, Weber André, Zuschratter Werner, Schraven Burkhart, Simeoni Luca, Müller Andreas J
Abstract excerpt
The enzymatic activity of the Src family tyrosine kinase p56Lck (Lck) is tightly controlled by differential phosphorylation of two tyrosine residues, Tyr394 and Tyr505 Phosphorylation of Tyr394 and the conformational opening of Lck are believed to activate the kinase, whereas Tyr505 phosphorylation is thought to generate a closed, inactive conformation of Lck. We investigated whether the conformation of Lck and...
Topics
- Blotting, Western
- Cell Membrane
- Cells, Cultured
- Humans
- Jurkat Cells
- Lymphocyte Activation
- Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
- Microscopy, Confocal
- Microscopy, Fluorescence